As in previous Metroid games, Metroid Prime takes place in a large, open-ended world in which regions are connected by elevators. Each region has a set of rooms separated by doors that can be opened with a shot from the correct beam. The gameplay involves solving puzzles to reveal secrets, platform jumping, and shooting foes with the help of a "lock-on" mechanism that allows circle strafing while staying aimed at the enemy. Metroid Prime is the first game in the Metroid series to use a first-person view instead of side-scrolling, except in Morph Ball mode, when Samus' suit transforms into an armored ball and the game uses a third-person camera.
The protagonist, Samus Aran, must travel through the world of Tallon IV searching for twelve Chozo Artifacts that will open the path to the Phazon meteor impact crater, while collecting power-ups that enable the player to reach previously inaccessible areas. The Varia Suit, for example, protects Samus' armor against dangerously high temperatures, allowing her to enter volcanic regions. Some of the items are obtained after boss and mini-boss fights, which are encountered in all regions except Magmoor Caverns. Items must be collected in a specific order so that the player may progress. For example, players cannot access certain areas until they find a certain Beam to open doors, or discover new ordnance with which to beat bosses. Like the rest of the series, players are incentivized to explore the open world to find upgrades such as ammunition packs and extra health